Translational_Unit

Part:BBa_K497009

Designed by: Joanna Hare   Group: iGEM10_Macquarie_Australia   (2010-10-26)

Agrobacterium tumefaciens bacteriophytochrome with RBS

The Agrobacterium tumefaciens bacteriophytochrome binds to biliverdin, a chromophore. Biliverdin is produced in the heme metabolic pathway catalysed by heme oxygenase. Once bound to the chromophore the complex can absorb red and far red light causing the complex to change colour from blue to green reversibly. A ribosome binding site has been added upstream. Unlike BBa_K497004 this part does not include the 3' transcriptional terminator. Sequence from National Center for Biotechnology Information

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1660
    Illegal PstI site found at 2056
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1660
    Illegal PstI site found at 2056
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1863
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1660
    Illegal PstI site found at 2056
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1660
    Illegal PstI site found at 2056
    Illegal NgoMIV site found at 1192
    Illegal NgoMIV site found at 1690
    Illegal AgeI site found at 1755
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None