Translational_Unit

Part:BBa_K4951072

Designed by: Julia Vu   Group: iGEM23_Stanford   (2023-10-11)


pTwist Amp Med Copy mStayGold + LacZ - Insert (Switch)

The pTwist mStayGold plasmid with a LacZ operon was designed to have an inducible switch, that would only express the mStayGold signal when the operon is activated and the switch is in the presence of the trigger plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 263
    Illegal EcoRI site found at 955
    Illegal XbaI site found at 236
    Illegal PstI site found at 224
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 263
    Illegal EcoRI site found at 955
    Illegal PstI site found at 224
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 263
    Illegal EcoRI site found at 955
    Illegal BamHI site found at 242
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 263
    Illegal EcoRI site found at 955
    Illegal XbaI site found at 236
    Illegal PstI site found at 224
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 263
    Illegal EcoRI site found at 955
    Illegal XbaI site found at 236
    Illegal PstI site found at 224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 608
    Illegal SapI.rc site found at 23


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