RNA

Part:BBa_K4950013

Designed by: Lucas Ng   Group: iGEM23_PennState   (2023-10-12)


BThrom2 Riboswitch Insert

Riboswitch sequence (2nd design) intended to bind to permit the translation of the downstream mRFP1 gene when bound to bovine thrombin (BThrom). Sequence is intended to be inserted between promoter and protein CDS beginning with the first eight nucleotides of the mRFP1 gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal PstI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal PstI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal PstI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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