Translational_Unit

Part:BBa_K4912009

Designed by: Jiang Yunchong   Group: iGEM23_HUBU-HUBEI-CHINA   (2023-10-10)


Key enzymes for synthesizing hydroxytyrosol

KivD and hpaBC are two key enzymes in the synthesis pathway of hydroxytyrosol. If kivD is overexpressed, it can lead to the accumulation of toxic substance phenylacetaldehyde, exacerbating the metabolic burden on cells; If the expression of hpaBC is insufficient, it will lead to the accumulation of tyrosol and low production efficiency of HT. It can be seen that the balanced expression of kivD and hpaBC is a key factor affecting the production of hydroxytyrosol.

We designed to use strong promoters PbacA, Pbay derived from Bacillus licheniformis and P43 from Bacillus subtilis to optimize the expression intensity of kivD and hpaBC.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1143
    Illegal PstI site found at 2404
    Illegal PstI site found at 3745
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1143
    Illegal PstI site found at 2404
    Illegal PstI site found at 3745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1143
    Illegal BglII site found at 99
    Illegal BglII site found at 919
    Illegal BglII site found at 2095
    Illegal BglII site found at 2420
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1143
    Illegal PstI site found at 2404
    Illegal PstI site found at 3745
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1143
    Illegal PstI site found at 2404
    Illegal PstI site found at 3745
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4200


[edit]
Categories
//chassis/prokaryote
Parameters
biology