RNA

Part:BBa_K4899001

Designed by: Morten Pedersen Hauger   Group: iGEM23_UiOslo-Norway   (2023-10-04)


miniaturized CAS12f guide RNA

a miniaturized sgRNA for Cas12f that targets a DNA sequence containing the sequence of the micro RNA miR199b outside the miR199b sequence.

Usage and Biology

A sgRNA for cas12f and dcasMINI (and CasMINI) that has been subjected to hairpin truncation to reduce its size, it was created by the fusion of the tracrRNA and crRNA used in the generation of cas12fs natural guide RNA (Xu et.al). It serves to target the site of cleavage and strongest binding for cas12f and dCasMINI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None