RNA
ASTERISK

Part:BBa_K4877000

Designed by: Saint Fischer   Group: iGEM23_Bielefeld-CeBiTec   (2023-10-05)


ASTERISK: ADAR based precise detection and expression tool

Based on Gayet, R.V., Ilia, K., Razavi, S. et al. (2023), we (Bielefeld-CeBiTec 2023) developed a modified DART VADAR (Detection and Amplification of RNA Triggers via ADAR) system. Our Part will allow future iGEM-Teams to access a highly specific, solely mRNA based sensing and expression platform.

We designed this part to be as accessible as possible for future projects and we believe many projects will be able to profit from our Part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 826
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 775
    Illegal BamHI site found at 1085
    Illegal XhoI site found at 818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2114


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Categories
Parameters
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