Composite

Part:BBa_K4864011

Designed by: Jyothishree V   Group: iGEM23_SVCE-CHENNAI   (2023-10-10)


pAlx-RBS-GFP

This part consists of a pH-inducible promoter, and a reporter gene. The pH-inducible promoter, Alx (which stands for Alkaline Xylanase), gets activated in the pH range above 7. In order to confirm the working of the Alx promoter at the laboratory level, we added the reporter gene (GFP).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 312
    Illegal BsaI.rc site found at 1055


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Categories
Parameters
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