Composite

Part:BBa_K4864009

Designed by: Jyothishree V   Group: iGEM23_SVCE-CHENNAI   (2023-10-10)


pAlx-RBS-Cry2Aa-Linker-GFP

This part consists of a pH-inducible promoter, a Cry2Aa-coding sequence, a Tandem GS linker, and a reporter gene. The pH-inducible promoter, Alx (which stands for Alkaline Xylanase), gets activated in the pH range above 7. In order to confirm the secretion of the Cry2Aa protein at the laboratory level, we added the reporter gene (GFP) in fusion with the laccase gene using a 13 Tandem GS linker.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 772
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 772
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1138
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 772
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 772
    Illegal AgeI site found at 567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1218
    Illegal BsaI.rc site found at 312
    Illegal BsaI.rc site found at 700
    Illegal BsaI.rc site found at 2978
    Illegal SapI site found at 879
    Illegal SapI site found at 939


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Categories
Parameters
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