Part:BBa_K4811033

TMS(Mn2)

Long description

This is a transriptional unit for a tRNA mimicking structure (TMS), using the T7 promoter and terminator. In BL21(DE3), the T7 polymerase is under LacI control, meaning it will be expressed by IPTG, leading to expression of the TMS RNA. The TMS is a novel piece of synthetically designed RNA, which folds in the same manner as tRNA, developed by Paul et al. It is a transencoded genetic switch, which binds to the Ribosome Binding Site(RBS) BBa_K4811001, repressing translation. The binding regions are flanking the RBS, and no binding happens in the RBS consensus sequence. This means that there are very few limitations on both the possible TMSs and repressible RBSs which could be engineered. Also, the D-loop of the TMS can be exchanged for additional functionality, such as an aptamer, making the TMS responsive to a ligand.

In this particular part, the D-loop has been exchanged for a Kanamycin-sensitive aptamer

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 105