RBS

Part:BBa_K4796003

Designed by: Oliver Hong   Group: iGEM23_UBC-Vancouver   (2023-09-10)


Strong 5'UTR for Vibrio natriegens (BBa_K3187014 derivative)

A 5'UTR/RBS for high levels of translation in Vibrio natriegens for use with the T7 expression system. Contains the T7 g10 leader sequence, a polyA tract and Shine-Dalgarno sequence derived from BBa_K3187014. A 5'UTR linker 3' of the SD sequence is derived the mdh gene of Bacillus methanolicus, taken from Tietze et al. (2022).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None