RNA

Part:BBa_K4757031

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-10-06)


sRNA MicC targeting BBa_J61100

Composite part from BBa_K4757021 and BBa_K4757028. This composite can be used for sRNA mediated gene repression by targeting the RBS BBa_J61100. The part was tested by expressing the sRNA with the XylS/Pm expression cassette and targeting the RBS (BBa_J61100) in front of mKate2 (BBa_K4757000), expressed by the constitutive promoter pEM7 and measuring the decrease in fluorescens. The repression strength was calculated by dividing the mean fluorescens after induction with the fluorescens of the genetic construct lacking the sRNA gene. Measurements were performed in P. fluorescens.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 88
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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