Coding
MC

Part:BBa_K4744777

Designed by: Julian Luka, Thalia von Nethen, Arno Schnizler   Group: iGEM23_Aachen   (2023-10-11)


mostcomm-Strep-17-LCI

Mostcomm-Strep-17-LCI is a construct containing our gene of interest, here "most common standard tag" BBa_K4744444 capable of binding lanthanites, a Strep-tag for purification and a 17x linker fused with an LCI anchor for immobilization on polystyrene.

This construct was expressed in our vector pET28a_mostcomm-Strep-17x-LCI.

For more information see our teams wiki: https://2023.igem.wiki/aachen/wetlab#assesspep

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//chassis/prokaryote/ecoli
//dna
Parameters
chassisE. coli BL21
functionAble to be expressed in E. coli BL21, purified through strep-mediated chromatography, immobilised on polystyrene using LCI, binds binding of trivalent lanthanoid-ions from heterogenous acidic solution