Part:BBa_K4735015
dmsA promoter driving superfolder GFP expression
dmsA promoter driving superfolder GFP expression. The part should promote expression of GFP under low oxygen conditions.
Testing indicates this does respond to oxygen. It shows no GFP fluorescence aerobically AND gives GFP fluorescence anaerobically at 0.5% oxygen and 2% oxygen in nitrogen.
This part is expected to only promote expression under low oxygen conditions. The composite part was grown in E. coli under 4 different oxygen conditions. Anaerobic, 0.5 % oxygen, 2% oxygen and fully aerobic (18% oxygen).
The graph shows how this promoter (Promoter C in the image) responded with 2 biological and 3 technical replicates per measurement point.
As can be seen this promoter responds to oxygen such that expression of superfolder GFP is higher under 3 oxygen deficient conditions, anaerobic, 0.5% and 2%, compared to fully aerobic growth conditions.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 142
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 142
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 142
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 195
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