Part:BBa_K4735014
NarK promoter fused to sfGFP
narK promoter driving superfolder GFP expression. The part should promote expression of GFP under low oxygen conditions.
Testing indicates this does respond very well to oxygen. It shows no GFP fluorescence anaerobically or aerobically but gives GFP fluorescence at 0.5% oxygen and 2% oxygen.
This part is expected to only promote expression under low oxygen conditions. The composite part was grown in E. coli under 4 different oxygen conditions. Anaerobic, 0.5 % oxygen, 2% oxygen and fully aerobic (18% oxygen).
The graph shows how this promoter (Promoter B in the image) responded with 2 biological and 3 technical replicates per measurement point.
As can be seen this promoter responds to oxygen such that expression of superfolder GFP occurs at oxygen concentrations of 0.5% and 2% but not either fully anaerobically or under fully aerobic growth conditions.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 143
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 143
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 143
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 196
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