Composite

Part:BBa_K4729708

Designed by: Yasoo Morimoto   Group: iGEM23_Marburg   (2023-10-10)


Ptau + VirG TiBo542 N80D + BBR1

a

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 186
    Illegal PstI site found at 1415
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1415
    Illegal NotI site found at 606
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1873
    Illegal BamHI site found at 2049
    Illegal BamHI site found at 2341
    Illegal XhoI site found at 614
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 186
    Illegal PstI site found at 1415
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 186
    Illegal PstI site found at 1415
    Illegal NgoMIV site found at 435
    Illegal NgoMIV site found at 1223
    Illegal NgoMIV site found at 2186
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Khan, S. R., Gaines, J., Roop, R. M., II, & Farrand, S. K. (2008). Broad-Host-Range Expression Vectors with Tightly Regulated Promoters and Their Use To Examine the Influence of TraR and TraM Expression on Ti Plasmid Quorum Sensing. In Applied and Environmental Microbiology (Vol. 74, Issue 16, pp. 5053–5062). American Society for Microbiology. https://doi.org/10.1128/aem.01098-08

Seiler, V. N. (2023). Improved inducible switches for the implementation of genetic tools in Sinorhizobium meliloti and Ensifer adhaerens (Master's thesis, Philipps-University Marburg)


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