Plasmid

Part:BBa_K4706019

Designed by: Niklas Küppers   Group: iGEM23_Duesseldorf   (2023-10-01)


pSB1C30YIpHR-HO SrpR-PHO5 phosphate dependent cell death

pSB1C30YIpHR-HO SrpR-PHO5 phosphate dependent cell death

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3049
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3049
    Illegal BglII site found at 2500
    Illegal BglII site found at 4476
    Illegal BglII site found at 4824
    Illegal BamHI site found at 3613
    Illegal XhoI site found at 1533
    Illegal XhoI site found at 2425
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3049
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3049
    Illegal XbaI site found at 3064
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3597


[edit]
Categories
//cds/lysis
//chassis/eukaryote/yeast
//chassis/prokaryote/ecoli
//dna/chromosomalintegration
//function/celldeath
Parameters
None