Part:BBa_K4696005
2x Kink Turn (2x K-Turn) RNA Motif
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 54
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 60
- 1000COMPATIBLE WITH RFC[1000]
Overview
Kink turns (k-turns) are an RNA structural motif that consists of a helix-internal loop-helix motif that introduces a very tight conformational change. This 2x K-Turn seqeunces is recognised by RNA binding protein L7Ae which when bound induces this strong confromational change on the RNA preventing downstream translation.
Usage and Biology
Kink turns (K-Turns) are a prevalent structural motif in RNA, consisting of a sharp turn into a helical axis that provides architectural stability and a binding site for various proteins (Huang & Lilley, 2016; Schroeder et al., 2010). A K-Turn variation found in box C/D RNA has high binding affinity to the L7Ae protein (BBa_K3113009), which induces a sharp conformational change in the RNA that blocks downstream translation, enabling precise and togglable regulation of downstream expression (Saito et al., 2009).
References
Huang, L., & Lilley, D. M. (2016). The kink turn, a key architectural element in RNA structure. Journal of Molecular Biology, 428(5), 790–801. https://doi.org/10.1016/j.jmb.2015.09.026
Saito, H., Kobayashi, T., Hara, T., Fujita, Y., Hayashi, K., Furushima, R., & Inoue, T. (2009). Synthetic translational regulation by an L7Ae–kink-turn RNP Switch. Nature Chemical Biology, 6(1), 71–78. https://doi.org/10.1038/nchembio.273
Schroeder, K. T., McPhee, S. A., Ouellet, J., & Lilley, D. M. (2010). A structural database for k-turn motifs in RNA. RNA, 16(8), 1463–1468. https://doi.org/10.1261/rna.2207910
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