RNA

Part:BBa_K4662020

Designed by: Julia Wyss   Group: iGEM23_UZurich   (2023-10-02)


crRNA to block Beta-galactosidase

crRNA designed to block Beta-galactosidase when tracrRNA is added and expressed with dCas9.


Characterization

Gene Location: NC_000913.3:365452

PAM sequence on antisense strand

Preddicted efficiency: 70%


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

For more information on it usage BBa_K4662049

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Categories
Parameters
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