RNA

Part:BBa_K4662011

Designed by: Julia Wyss   Group: iGEM23_UZurich   (2023-10-02)


crRNA to block Pyruvate formate-lyase 1-activating enzyme

crRNA designed to block the promoter of Pyruvate formate-lyase 1-activating enzyme when tracrRNA is added and expressed with dCas9.


Characterization

Gene Location NC_000913.3:950938

PAM sequence on antisense strand

Predicted efficiency: 77%

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Part can be used to manipulate central metabolism leading to more available pyruvate in the cell.

For more information on it's usage BBa_K4662051



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