RNA
Spacer1

Part:BBa_K4654009

Designed by: Ronja Friedhoff, Felix Jarecki   Group: iGEM23_TU-Braunschweig   (2023-10-08)


Spacer Sequence 1

The Spacers are for maintaining the natural environment of the riboswitch (BBa_K4654011). This is crucial because it influences the folding and functionality of the riboswitch. We therefore made the decision to retain the spacer sequence between the promoter and the riboswitch as this is crucial for the preservation of the integrity and functionality of the riboswitch.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rna
Parameters
device_typespacer
functionPreserves the natural environment of BBa_K4654011