RBS

Part:BBa_K4632013

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-09)


BBa_B0032X（medium）

RBS32X is mutated from RBS32. An ideal mutant RBS 32X (the last two core bases were mutated into GA) was screened by fluorescence, The transcription intensity of RBS32X is medium. source: [1].

part-7-1.png

Fig.1 Expression optimization of the mutational las systems.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Jiang W, He X, Luo Y et al. Two Completely Orthogonal Quorum Sensing Systems with Self-Produced Autoinducers Enable Automatic Delayed Cascade Control[J]. ACS SYNTH BIOL, 2020,9(9):2588-2599.



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