Part:BBa_K4597003
hGIF with TIR and signal peptide - E.coli
Full protein coding sequence, translation initiation region (TIR), and signal peptide sequence (PelB) for human gastric intrinsic factor (GIF) expression in Escherichia coli while in the human gut. The gene sequence has been codon optimized for expression in E. coli with an evolved signal peptide on the N-terminal to optimize the export of the protein to the periplasm for proper folding. A TIR sequence is upstream of the gene and signal sequence (the start of the signal peptide gene sequence is important for efficient translation and is part of the TIR). Restriction sites can be added to both ends of the gene sequence to facilitate cloning, we used Bsa-I HFv2 restriction sites for Golden Gate assembly with NEB's Golden Gate kit, which included an appropriate vector, pGGAselect.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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