RNA
gRNA-mIGK

Part:BBa_K4574040

Designed by: Alessio D. Nahmad   Group: iGEM23_Munich   (2023-10-06)


RNA guide mIGK

CRISPR guide RNA (gRNA), designed to target the mouse IGK locus, precisely the light chain kappa constant region (Lκ). When used with the CRISPR/Cas9 system, this guide sequence, 5'-TGGTGCAGCATCAGCCC^CTG(AGG)-3', ensures precise double-stranded break 3 bp upstream of the PAM recognition sequence (indicated in parentheses).

Source

The following basic part was designed to target genetic locus[1].

Organism Mus musculus (house mouse)
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Gene IGK: immunoglobulin kappa chain complex (NCBI 243469)
Location 6 C1, 70703413←70703435

It was first identified and used by Nahmad et al.[2].

Usage and Biology

This basic part has can be used as a component of a CRISPR knock-out system in mouse B-cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  1. ↑ Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=243469
  2. ↑ Barzel, A., Nahmad, A. D., Akriv, T., Fried, M., Dotan, I., & Nataf, D. (2020). Genetic engneering of B cell receptors and uses thereof in antigen-induced antibody secretion (World Intellectual Property Organization Patent WO/2020/053865). https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2020053865


[edit]
Categories
Parameters
None