RNA
gRNA-mIGH

Part:BBa_K4574039

Designed by: Alessio D. Nahmad   Group: iGEM23_Munich   (2023-10-06)


RNA guide mIGH

CRISPR guide RNA (gRNA), designed to target the mouse IGH locus, precisely between the J4 joining segment gene and the intronic enhancer Eμ regulatory element. When used with the CRISPR/Cas9 system, this guide sequence, 5'-CGATGCATAGGGACAAA^GAG(TGG)-3', ensures precise double-stranded break 3 bp upstream of the PAM recognition sequence (indicated in parentheses).

Source

The following basic part was designed to target genetic locus[1].

Organism Mus musculus (house mouse)
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Gene IGH: immunoglobulin heavy chain complex (NCBI 111507)
Location 12 F1-F2, 113391736←113391755

It was first identified and used by Nahmad et al.[2].

Usage and Biology

This basic part can used as a component of a CRISPR homology-directed repair system in mouse B-cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  1. ↑ Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=111507
  2. ↑ Barzel, A., Nahmad, A. D., Akriv, T., Fried, M., Dotan, I., & Nataf, D. (2020). Genetic engneering of B cell receptors and uses thereof in antigen-induced antibody secretion (World Intellectual Property Organization Patent WO/2020/053865). https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2020053865


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