RNA
gRNA-hIGK2

Part:BBa_K4574037

Designed by: Christopher Pfeiffer   Group: iGEM23_Munich   (2023-10-06)


RNA guide hIGK-2

CRISPR guide RNA (gRNA), designed to target the human IGK locus, precisely the light chain kappa constant region (Lκ). When used with the CRISPR/Cas9 system, this guide sequence, 5'-CCTGGGAGTTACCCGAT^TGG(AGG)-3', ensures precise double-stranded break 3 bp upstream of the PAM recognition sequence (indicated in parentheses).

Source

The following basic part was designed to target genetic locus[1].

Organism Homo sapiens (human)
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Gene IGK: immunoglobulin kappa locus (NCBI 50802)
Location 2p11.2, 88857525→88857547

Usage and Biology

This basic part has been used as a component of a CRISPR knock-out system in RAMOS B-cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  1. ↑ Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=50802


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