RNA
gRNA-hIGH2

Part:BBa_K4574034

Designed by: Christopher Pfeiffer   Group: iGEM23_Munich   (2023-10-06)


RNA guide hIGH-2

CRISPR guide RNA (gRNA), designed to target the human IGH locus, precisely between the J6 joining segment gene and the intronic enhancer Eμ regulatory element. When used with the CRISPR/Cas9 system, this guide sequence, 5'-CCCTGCTCTCATCAAGA^CCG(GGG)-3', ensures precise double-stranded break 3 bp upstream of the PAM recognition sequence (indicated in parentheses).

Source

The following basic part was designed to target genetic locus[1].

Organism Homo sapiens (human)
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Gene IGH: immunoglobulin heavy locus (NCBI 3492)
Location 14q32.33, 105862710→105862729

Usage and Biology

This basic part has been used as a component of a CRISPR homology-directed repair system in RAMOS B-cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  1. ↑ Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=3492


[edit]
Categories
Parameters
None