Part:BBa_K4397000
Amyloid Beta aptamer: Beta-55
Amyloid Beta aptamer
Target: Aβ40 fibril
Binding affinity: 29 nM (affinity chromatography)
Usage and Biology
β55 is a novel RNA aptamer generated from a SELEX screen against monomeric Aβ1–40 was previously shown to bind synthetic amyloid fibrils [1]. Despite that SELEX screens have been used against covalently stabilized Aβ trimmers, Aβ1–40 conjugated to colloidal gold nanoparticles as well as DNA aptamer selected against α-synuclein, no ex vivo stains of human AD brain tissues have been performed with aptamer probes [1]. Therefore, the novel ability of β55 to bind amyloid plaques in ex vivo human AD brain tissue and in live transgenic mouse models of AD is of great importance [1]. The main idea behind this aptamer is that β55 binds Aβ oligomers and is able to visualize the oligomeric halo surrounding the dense cores of amyloid plaques [1].
[1] Farrar, C. T., William, C. M., Hudry, E., Hashimoto, T., & Hyman, B. T. (2014). RNA aptamer probes as optical imaging agents for the detection of amyloid plaques. PLoS One, 9(2), e89901.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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