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Coding
Part:BBa_K4384010
Designed by: Zike Yao Group: iGEM22_HK_SSC (2022-10-09)
5YSS_Y152V_W184Y
This cyclase is expected to convert the molecule 5AVA to valerolactam. This cyclase is modified by our team by mutating the 152th residue tyrosine to valine, and the 184th residue tryptophan to tyrosine. This cyclase is found present in Citrobacter freundii. [1]
references: [1] Yeom, S.-J.; Kim, M.; Kwon, K. K.; Fu, Y.; Rha, E.; Park, S.-H.; Lee, H.; Kim, H.; Lee, D.-H.; Kim, D.-M.; Lee, S.-G. A Synthetic Microbial Biosensor for High-Throughput Screening of Lactam Biocatalysts. Nature Communications 2018, 9 (1). https://doi.org/10.1038/s41467-018-07488-0.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 10
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |