Coding

Part:BBa_K4384010

Designed by: Zike Yao   Group: iGEM22_HK_SSC   (2022-10-09)


5YSS_Y152V_W184Y

This cyclase is expected to convert the molecule 5AVA to valerolactam. This cyclase is modified by our team by mutating the 152th residue tyrosine to valine, and the 184th residue tryptophan to tyrosine. This cyclase is found present in Citrobacter freundii. [1]

references: [1] Yeom, S.-J.; Kim, M.; Kwon, K. K.; Fu, Y.; Rha, E.; Park, S.-H.; Lee, H.; Kim, H.; Lee, D.-H.; Kim, D.-M.; Lee, S.-G. A Synthetic Microbial Biosensor for High-Throughput Screening of Lactam Biocatalysts. Nature Communications 2018, 9 (1). https://doi.org/10.1038/s41467-018-07488-0.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None