Part:BBa_K4384005

5YSS_Y152A

This cyclase is expected to convert the molecule 5AVA to valerolactam. This cyclase is modified by our team by mutating the 152th residue tyrosine to alanine. We have modified the cyclase as it was reported that Y152A mutant showed the highest cyclization activity, with a three-fold increase, for 6-ACA, which indicates that it may also show the highest cyclization activity for 5AVA. This cyclase is found present in Citrobacter freundii. [1]

reference: [1] Yeom, S.-J.; Kim, M.; Kwon, K. K.; Fu, Y.; Rha, E.; Park, S.-H.; Lee, H.; Kim, H.; Lee, D.-H.; Kim, D.-M.; Lee, S.-G. A Synthetic Microbial Biosensor for High-Throughput Screening of Lactam Biocatalysts. Nature Communications 2018, 9 (1). https://doi.org/10.1038/s41467-018-07488-0

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 10
  
• 1000
  COMPATIBLE WITH RFC[1000]