Part:BBa_K4382010

An endoxylanase - XynA

Usage and Biology

Xylanase A(XynA - Uniprot ID P18429) is an endo- 1,4 - beta xylanase. This enzyme catalyses the breakdown of xylan into xylo-oligosaccharides.

This is an extracellular enzyme, i.e. the enzyme is secreted out of the cell for the reaction to occur. It helps breakdown xylan into xylo-oligosaccharides - namely xylobiose, xylotriose and MeGaX4(methylglucuronoxylotetraose). A small amount of xylose is also produced in the process.

Degradation of hemicellulose enhances the rate of degradation of cellulose. This acts in synergy with exoglucanases to facilitate complete breakdown of xylans.

References

1. Rhee, M. S., Wei, L., Sawhney, N., Rice, J. D., St. John, F. J., Hurlbert, J. C., & Preston, J. F. (2014, February). Engineering the Xylan Utilization System in Bacillus subtilis for Production of Acidic Xylooligosaccharides. Applied and Environmental Microbiology, 80(3), 917–927. https://doi.org/10.1128/aem.03246-13

2. St. John, F. J., Rice, J. D., & Preston, J. F. (2006, December 15). Characterization of XynC from            Bacillus subtilis            subsp.            subtilis            Strain 168 and Analysis of Its Role in Depolymerization of Glucuronoxylan. Journal of Bacteriology, 188(24), 8617–8626. https://doi.org/10.1128/jb.01283-06

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 80
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 85
  Illegal PstI site found at 80
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 80
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 80
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 489
  Illegal SapI.rc site found at 543