RBS

Part:BBa_K4382009

Designed by: UZAIR MOTIWALA   Group: iGEM22_IISER_Bhopal   (2022-10-09)


RBS for Bacillus Subtilis

It is an efficient RBS used in Bacillus Subtilis for protein expression.For our project we chose this RBS because it is present in the pCri-18a plasmid backbone adjacent to Pgrac promoter(BBa_K1074012).

References

1)Goulas, T., Cuppari, A., Garcia-Castellanos, R., Snipas, S., Glockshuber, R., Arolas, J. L., & Gomis-Rüth, F. X. (2014). The PCRI system: A vector collection for recombinant protein expression and purification. PLoS ONE, 9(11). https://doi.org/10.1371/journal.pone.0112643

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
//rbs/prokaryote/constitutive/miscellaneous
//regulation/constitutive
//ribosome/prokaryote/bsubtilis
Parameters
None