Part:BBa_K4382007

Pectin lyase - PelA

Usage

The PelA gene is a pectin lyase - which is useful for breaking down the pectin backbone. Sourced from the soil bacterium Bacillus subtilis 7-3-3, this particular lyase was first isolated by Zou et al in 2013. The isolation of this pectinase adds to the ongoing effort of characterizing new enzymes for industrial use. The application of this bacterial enzyme which has been shown in the above research paper is degumming of plant matter - which we aim to extrapolate in our project; by degrading the pectin backbone in crop stubble

Biology

PelA isolated from Bacillus subtilis 7-3-3 works optimally at a temperature of 55 degrees Celsius and at a pH of 9.5 (the latter feature makes it an alkaline pectin lyase). The enzyme shows higher stability for longer incubation periods at moderate temperatures, whereas at 60-70 degrees Celsius, longer incubations drastically decrease the stability.

A process called degumming is a process by which non-cellulosic parts are separated from cellulosic fibers - which is of commercial importance. A plant called Ramie was treated with recombinant Pel A in order to check its ability to degum ramie. It was found that Ramie was degummed without any kind of pretreatment of the ramie (which otherwise would involve harsh treatments - like using boiling water with or without salt solution) - thus acting as an efficient solution.

References

1. Zou, M., Li, X., Shi, W., Guo, F., Zhao, J., & Qu, Y. (2013, August). Improved production of alkaline polygalacturonate lyase by homologous overexpression pelA in Bacillus subtilis. Process Biochemistry, 48(8), 1143–1150. https://doi.org/10.1016/j.procbio.2013.05.023

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 570
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 674
  Illegal BsaI site found at 1048
  Illegal BsaI site found at 1153