Part:BBa_K4370011
sgRNA_lsr2A_STREPTO
This part encodes a single guide RNA targeting one of the lsr2 paralogs ( lsr2A ) encoding the main xenogeneic silencer of Streptomyces . It has been designed to target different strains such as S. ambofaciens ATCC 23877, S. bottropensis ATCC 25435, S. venezuelae ATCC 10712 and S. rimosus ATCC 10970.
Usage and Biology
Xenogeneic silencers of Lsr2 family have been shown to silence the expression of specialized metabolite biosynthetic gene clusters (Gerke et al., eLife, 2019 ; Zhang et al., Genetics and Molecular Biology, 2021 ; Deng et al., RSC Adv, 2017). Thus decreasing their expression has been associated with increased production of specialized metabolites including antibiotics.
We have engineered genetic tools via the Crispr-dCas9 system to switch off lsr2a and force antibiotic production in a low carbon environment.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Emma J Gehrke Xiafei Zhang Sheila M Pimentel-Elardo Andrew R Johnson Christiaan A Rees Stephanie E Jones Hindra Sebastian S Gehrke Sonya Turvey Suzanne Boursalie Jane E Hill Erin E Carlson Justin R Nodwell Marie A Elliot, “Silencing cryptic specialized metabolism in Streptomyces by the nucleoid-associated protein Lsr2” (2019) eLife 8:e47691
https://doi.org/10.7554/eLife.47691
Xiafei Zhang, Sara N. Andres, Marie A. Elliot, “Interplay between Nucleoid-Associated Proteins and Transcription Factors in Controlling Specialized Metabolism in Streptomyces” (2021) mBio Vol. 12, No. 4
https://doi.org/10.1128/mBio.01077-21
Lina Deng,a Rui Wang,a Guowei Wang,*a Mingxu Liu,a Guojian Liao,a Zhihua Liaob and Min Chen, “Targeted isolation of sulfur-containing metabolites from Lsr2-deletion mutant strain of Streptomyces roseosporus” (2017) RSC Adv., 2017,7, 37771-37777
https://doi.org/10.1039/C7RA06482A
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