Part:BBa_K4365020

turboRFP (yeast)

Turbo Red Fluorescent Protein (turboRFP) is an orange fluorescent protein isolated from Entacmaea quadricolor with excitation and emission maxima 553 and 574 respectively.It possesses prolonged fluorescence lifetime and high pH-stability, and is fast-maturing TurboRFP is more than twice brighter than DsRed2 ^[1].

It could be used as a selection marker or used for protein detection if fused with another protein. This sequence is codon optimized for expression in Saccharomyces cerevisiae.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 683

Usage and Biology

Picture of the sea anemone Entacmaea quadricolor.

Emission (EM) and Excitation (EX) spectra of turboRFP. Excitation maximum = 553 nm. Emission maximum = 574 nm. FP database. 187 % brighter than of EGFP.

↑ Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062

[1] Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062

[1]