Composite

Part:BBa_K4345024

Designed by: Luka Van den Berghe   Group: iGEM22_KU_Leuven   (2022-10-08)


5' UTR of prfA with ccdA fused to sfGFP

Design of a thermoregulated translation of antitoxin ccdA. Fluorescent protein sfGFP was linked to the antitoxin with a rigid linker for visualisation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1229
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

The figure below shows the secondary structure of the RNA thermometer.

PrfA Johansson et al 2002.jpeg

Image obtained from Johansson et al., 2002

References

Johansson, J., Mandin, P., Renzoni, A., Chiaruttini, C., Springer, M., & Cossart, P. (2002, September). An RNA Thermosensor Controls Expression of Virulence Genes in Listeria monocytogenes. Cell, 110(5), 551–561. https://doi.org/10.1016/s0092-8674(02)00905-4

[edit]
Categories
//biosafety/kill_switch
//cds/reporter/gfp
//regulation/constitutive
//rna/riboswitch
Parameters
biology
control
emission
excitation