Translational_Unit

Part:BBa_K4310019

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


Cold inducible promoter + Cre recombinase

CAMV 35S - loxP - mCherry - Tnos - pCnAFP - Kozak - Cre - Tnos - loxP - Kozak - eGFP - Tnos

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 830
    Illegal PstI site found at 1929
    Illegal PstI site found at 2788
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6697
    Illegal PstI site found at 830
    Illegal PstI site found at 1929
    Illegal PstI site found at 2788
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
    Illegal BglII site found at 1199
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 830
    Illegal PstI site found at 1929
    Illegal PstI site found at 2788
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 830
    Illegal PstI site found at 1929
    Illegal PstI site found at 2788
    Illegal NgoMIV site found at 4061
    Illegal NgoMIV site found at 4091
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1381
    Illegal BsaI site found at 3147
    Illegal BsaI site found at 4378
    Illegal BsaI site found at 7628
    Illegal BsaI.rc site found at 1660
    Illegal BsaI.rc site found at 4195
    Illegal BsaI.rc site found at 4657
    Illegal BsaI.rc site found at 5357
    Illegal BsaI.rc site found at 7907
    Illegal SapI site found at 1845
    Illegal SapI site found at 3098
    Illegal SapI site found at 4884
    Illegal SapI.rc site found at 3083
    Illegal SapI.rc site found at 3132
    Illegal SapI.rc site found at 4918


[edit]
Categories
Parameters
None