Translational_Unit

Part:BBa_K4310017

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


Cold inducible promoter + phiC31 recombinase

CAMV 35S - attB - mCherry - Tnos - pCnAFP - Kozak - phiC31 - Tnos - attP - Kozak - eGFP - Tnos

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 842
    Illegal PstI site found at 1941
    Illegal PstI site found at 2800
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3577
    Illegal NheI site found at 3747
    Illegal NheI site found at 4789
    Illegal NheI site found at 7501
    Illegal PstI site found at 842
    Illegal PstI site found at 1941
    Illegal PstI site found at 2800
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
    Illegal BglII site found at 1211
    Illegal BglII site found at 3235
    Illegal BamHI site found at 3972
    Illegal BamHI site found at 4002
    Illegal BamHI site found at 4088
    Illegal BamHI site found at 4098
    Illegal XhoI site found at 3929
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 842
    Illegal PstI site found at 1941
    Illegal PstI site found at 2800
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 842
    Illegal PstI site found at 1941
    Illegal PstI site found at 2800
    Illegal NgoMIV site found at 3169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1393
    Illegal BsaI site found at 5170
    Illegal BsaI site found at 8432
    Illegal BsaI.rc site found at 1672
    Illegal BsaI.rc site found at 5449
    Illegal BsaI.rc site found at 6161
    Illegal BsaI.rc site found at 8711
    Illegal SapI site found at 1857
    Illegal SapI site found at 3110
    Illegal SapI site found at 4459
    Illegal SapI site found at 5688
    Illegal SapI.rc site found at 3095
    Illegal SapI.rc site found at 3144
    Illegal SapI.rc site found at 4784
    Illegal SapI.rc site found at 5722


[edit]
Categories
Parameters
None