Translational_Unit

Part:BBa_K4310016

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


Heat shock inducible promoter + phiC31 recombinase

CAMV 35S - attB - mCherry - Tnos - pCvHSP70A - Kozak - phiC31 - Tnos - attP - Kozak - eGFP - Tnos

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 842
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2854
    Illegal NheI site found at 3024
    Illegal NheI site found at 4066
    Illegal NheI site found at 6778
    Illegal PstI site found at 842
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
    Illegal BglII site found at 1211
    Illegal BglII site found at 2512
    Illegal BamHI site found at 3249
    Illegal BamHI site found at 3279
    Illegal BamHI site found at 3365
    Illegal BamHI site found at 3375
    Illegal XhoI site found at 3206
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 842
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 842
    Illegal NgoMIV site found at 2228
    Illegal NgoMIV site found at 2446
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1393
    Illegal BsaI site found at 4447
    Illegal BsaI site found at 7709
    Illegal BsaI.rc site found at 1672
    Illegal BsaI.rc site found at 4726
    Illegal BsaI.rc site found at 5438
    Illegal BsaI.rc site found at 7988
    Illegal SapI site found at 1857
    Illegal SapI site found at 2387
    Illegal SapI site found at 3736
    Illegal SapI site found at 4965
    Illegal SapI.rc site found at 2372
    Illegal SapI.rc site found at 2421
    Illegal SapI.rc site found at 4061
    Illegal SapI.rc site found at 4999


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Categories
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