RNA

Part:BBa_K4251004

Designed by: Shi Lifang   Group: iGEM22_SubCat_Shanghai   (2022-09-21)


PDRG1-SiRNA-2

RDRG1-SiRNA-2

Contribution

Knock down the PDRG1 gene of the TMZ-resistance cell line and measure its resistance to TMZ

siRNA is a double-stranded non-coding RNA molecule, it usually between 20 and 24 base pairs, and it is also known as silencing RNA and short interfering RNA. siRNAs have very tight target specificity, this anti-sense strand binds to the target mRNA, and then the target mRNA cleavage is induced. We knocked down PDRG1 expression in glioma cells, established a stable PDRG1 knockdown cell line, and explored the effect of PDGR1 on glioma TMZ resistance. We tested its resistance to TMZ and the expression level of PDRG1 protein. The results of this study provide a basis for clinical research on TMZ-resistant glioma cell lines and provide a potential molecular drug target to reverse TMZ resistance.

Reference

1. Agnihotri, S., et al., Glioblastoma, a brief review of history, molecular genetics, animal models and novel therapeutic strategies. Arch Immunol Ther Exp (Warsz), 2013. 61(1): p. 25-41.

2. Luo, X., Y. Huang, and M.S. Sheikh, Cloning and characterization of a novel gene PDRG that is differentially regulated by p53 and ultraviolet radiation. Oncogene, 2003. 22(46): p. 7247-57.

3. Jiang, L., et al., PDRG1, a novel tumor marker for multiple malignancies that is selectively regulated by genotoxic stress. Cancer Biol Ther, 2011. 11(6): p. 567-73.

4. Tao, Z., et al., Knockdown of PDRG1 Could Inhibit the Wnt Signaling Pathway in Esophageal Cancer Cells. Ann Clin Lab Sci, 2019. 49(6): p. 794-803.

5. Tao, Z., et al., The PDRG1 is an oncogene in lung cancer cells, promoting radioresistance via the ATM-P53 signaling pathway. Biomed Pharmacother, 2016. 83: p. 1471-1477.

6. Xu, T. and D. Xiao, Oleuropein enhances radiation sensitivity of nasopharyngeal carcinoma by downregulating PDRG1 through HIF1alpharepressed microRNA-519d. J Exp Clin Cancer Res, 2017. 36(1): p. 3.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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