![](https://parts.igem.org/images/partbypart/icon_rna.png)
RNA
Part:BBa_K4244033
Designed by: Arthur Vancolen Group: iGEM22_Wageningen_UR (2022-10-08)
Switchable dSpycas9 sgRNA expression casset
A cassette, containing the SpeI promoter, a spacer and a sgrna scaffold. The spacer is flanked by two BsmBI recognition sites, so that new spacer can be easily introduced by digestion ligation.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 151
Illegal XbaI site found at 157
Illegal SpeI site found at 30
Illegal PstI site found at 169 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 151
Illegal NheI site found at 7
Illegal SpeI site found at 30
Illegal PstI site found at 169 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 151
Illegal BglII site found at 181 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 151
Illegal XbaI site found at 157
Illegal SpeI site found at 30
Illegal PstI site found at 169 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 151
Illegal XbaI site found at 157
Illegal SpeI site found at 30
Illegal PstI site found at 169 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |