RNA

Part:BBa_K4244033

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)


Switchable dSpycas9 sgRNA expression casset

A cassette, containing the SpeI promoter, a spacer and a sgrna scaffold. The spacer is flanked by two BsmBI recognition sites, so that new spacer can be easily introduced by digestion ligation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 151
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 151
    Illegal BglII site found at 181
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 151
    Illegal XbaI site found at 157
    Illegal SpeI site found at 30
    Illegal PstI site found at 169
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None