Translational_Unit

Part:BBa_K4215001

Designed by: Carmen Resnick   Group: iGEM22_UOregon   (2022-10-12)


RiboJ+RBS

This sequence containes a riboJ sequence designed to split mRNA, post-transcription, in order to divide a bimodal protein expression system under the control of a single promoter. The riboJ is followed by an RBS with a predicted translation initiation rate of 37086.88 (from DeNovo DNA salis calculator). This RBS translation rate is specific to the three-part complementation system and is designed to match the translation rate for the RBS directly following the promoter on pSEVA341 (item number BBa_K4215000). The sequence is golden gate compatible with BbsI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None