Protein_Domain

Part:BBa_K4190006

Designed by: Zoe L. Petroff   Group: iGEM22_UCSC   (2022-09-20)


Enterokinase Cut Site

Cut site for eventual removal of His-tag from Exendin-4 protein.

Usage and Biology

We included a four amino acid enterokinase protease cut site. This allows us to use enterokinase to cleave the his-tag from our protein once it is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/cleavage
Parameters
None