Translational_Unit
Part:BBa_K4187028
Designed by: Martin Pezous Group: iGEM22_Ionis_Paris (2022-10-11)
pBAD-RBS-mtrC-T7Te-T1Te
mtrC is a membrane cytochrome c-based protein expressed natively in Shewanella oneidensis. It carries out a function of extracellular electron transfer and was shown to improve electron Transfer to the bacteria surroundings in Dundas CM, Walker DJF, Keitz BK. Tuning Extracellular Electron Transfer by Shewanella oneidensis Using Transcriptional Logic Gates. ACS Synth Biol. 2020 Sep 18. We used this part to try to improve the electric output of our microbial fuel cell.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3038
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal PstI site found at 3038 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3038
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3038
Illegal AgeI site found at 979 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1480
Illegal SapI site found at 961
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Categories
Parameters
None |