Terminator

Part:BBa_K4152053

Designed by: Xiaoyan Yue   Group: iGEM22_TJUSLS_China   (2022-09-28)


AOX1 terminator

AOX1 terminator is the binding site of universal termination of for the transcriptional expression of methanol oxidase-1 expressed by AOX1. In our project, AOX1 terminator is located at the 3 'end of Protease K gene. On the one hand, when our linear vector is transferred into yeast cells, AOX1 terminator can recombine with AOX1 gene in yeast chromosome. On the other hand, in the presence of methanol, it acts as the terminator of Proteinase K expression to initiate transcription, so as to express our protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None