Part:BBa_K4147017

siRNA against the ODC gene

The ornithine decarboxylase (ODC) gene is a polyamine biosynthesis gene in Fusarium oxysporum, a pathogenic fungus responsible for causing chilli wilt. This gene is necessary for the pathogen's development, which is why it was chosen as a target.

Previous studies have been made to analyze the effect of silencing this gene. The results didn’t present any anomalies in the plant ODC gene expression, polyamine levels, or morphology, being highly specific against the spore phase of the fungal pathogen [1]. It has also been demonstrated that it is possible to transfer siRNAs from host plant cells the fungal cells of F. oxysporum [1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1]Singh, N., Mukherjee, S. K., & Rajam, M. V. (2020). Silencing of the ornithine decarboxylase gene of 0RW1S34RfeSDcfkexd09rT2fusarium oxysporum1RW1S34RfeSDcfkexd09rT2 f. sp. 0RW1S34RfeSDcfkexd09rT2lycopersici1RW1S34RfeSDcfkexd09rT2 by host-induced RNAi confers resistance to fusarium wilt in tomato. Plant Molecular Biology Reporter, 38(3), 419-429. doi:https://doi.org/10.1007/s11105-020-01205-2