Coding
PRO1

Part:BBa_K4139020

Designed by: Zachary Robinson & Jason Luddu   Group: iGEM22_Lethbridge   (2023-09-27)


Pro1

This construct begins with Pro1. Pro1 is a protein found in fungal and fungal-like pathogens including Plasmodiophora brassicae. After the Pro1 region we added a His-tag to make purification easier. The use of this part was to have other options other than PbEL04 in Plasmodiophora brassicae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 835
    Illegal PstI site found at 1135
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 835
    Illegal PstI site found at 1135
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1119
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 835
    Illegal PstI site found at 1135
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 835
    Illegal PstI site found at 1135
    Illegal NgoMIV site found at 1029
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter
Parameters
directionForward
proteinPro1
tagHis-Tag