Part:BBa_K4139018

CAPE-AFP

This construct begins with a His-tag, which helps with the purification of the protein. After the His-tag is our chimeric Anti-PbEL04 (CAPE) repeated three times, this was done to increase the change of binding with PbEL04 and overall structure to the chimeric fluorophore probe. Finally, we added a lysine rich region. The lysine rich region is present to bind to colloidal gold particles, which when excited, will fluoresce in visible light giving a signal which will be explored with the Club^2 test strips.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 157
  Illegal PstI site found at 328
  Illegal PstI site found at 499
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 157
  Illegal PstI site found at 328
  Illegal PstI site found at 499
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 157
  Illegal PstI site found at 328
  Illegal PstI site found at 499
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 157
  Illegal PstI site found at 328
  Illegal PstI site found at 499
  
• 1000
  COMPATIBLE WITH RFC[1000]