RBS

Part:BBa_K4054001

Designed by: Xinyan Han   Group: iGEM21_XJTLU-CHINA   (2021-10-01)


T7 RBS

efficient ribosome binding site from bacteriophage T7 gene 10.

Description

The key to cleavage is the presence of a primordial spacer adjacent motif (PAM) downstream of the target region. Cas9 is a double-stranded (DS)DNA endonuclease that uses crRNA guidance to determine the cleavage site. During the processing of the crRNA precursor, acrRNA guide is loaded on Cas9, requiring the precursor (tracrRNA) and a small RNA antisense of RNAse III.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None