RNA

Part:BBa_K4050019

Designed by: Kelly Styles   Group: iGEM21_Victoria_Wellington   (2021-09-30)


20 nt spacer sgRNA for spermidine synthase gene in S. elongatus UTEX 2973

20 nt spacer for an sgRNA to recruit Cas9 to the spermidine synthase gene (speE - CP006471.1:2636619..2637479) in Synechococcus elongatus UTEX 2973 to perform a double strand break. This can then increase efficiency of targeted knock-out or knock-in events at this locus. Can be used with flanking regions of the speE gene (BBa_K4050017 & BBa_K4050018) to knock-out the speE gene in Synechococcus elongatus UTEX 2973.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None