RBS

Part:BBa_K4017002

Designed by: César Daniel Marques Duarte   Group: iGEM21_NOVA_LxPortugal   (2021-09-30)


Ribosome Binding Site

This part encodes for the binding region of bacterial ribosomes in mRNA and ensures the translation of both the signal peptides used by the team (BBa_K4017003 - BBa_K4017007) and the proteins for plastic degradation PETase and MHETase (BBa_K4017010 - BBa_K4017013). The Ribosome Binding Site (RBS) is a rich A/G region, upstream of the start codon ATG of an mRNA. Translation initiation in bacteria requires RBS, as it is responsible for the recruitment of the small bacterial ribosome (30S) and the translation initiation [1-2].


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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